‘凯特’与‘新世纪’杏自交不亲和S-RNase基因的检测及克隆
2006-10-30分类号：S662.2
【部门】山东农业大学果树生物学实验室 石河子大学园艺系石河子832003 山东农业大学果树生物学实验室 山东农业大学果树生物学实验室 泰安271018 泰安271018 泰安271018
【摘要】Frozen young leaves of apricot(Armeniaca vulgaris) ‘Katy’ and ‘Xinshiji' were used for isolation of total DNA. Total RNA was isolated from their styles at the balloon stage. DNA and cDNA were amplified through PCR using AS1 Ⅱ and ArmyC5R as primers designed according to the conserved (C1,C5) sequenc...
【关键词】杏 自交不亲和 S-RNase基因 序列
【基金】国家自然科学基金项目(30370992)。
【所属期刊栏目】林业科学
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